The painting was previously shown in the 2021 landmark exhibition Children of the Renaissance. This exhibition was curated by Dr. Samuel Mareel and was nominated for the International Exhibition of the Year by the Museum+Heritage Awards. Until recently, it belonged to a private collection and was brought to market through the British art dealer James Macdonald Fine Art.

This work is associated with a second portrait, which may depict an older sibling. Together, the two paintings reveal strong iconographic connections with other Habsburg family portraits, such as the triptych of Eleonora, Charles V, and Isabella attributed to the Master of the Mechelen Saint George’s Guild (Kunsthistorisches Museum, Vienna), as well as examples from the Royal Collection. A detailed examination and further research will follow this important acquisition.